Evaluating the influence of phenolic compounds to gut bacteria-host cell interaction via HPLC-MS/MS based targeted metabolic profiling

Abstract

This study evaluated the influence of phenolic compounds and their influence to the interaction of human gut bacteria and host cells. Four bacterial stains were studied in this project. The minimum inhibitory concentration of phenolic compounds on these four strains were investigated. The effects of phenolic compounds on adhesion of bacteria to cultured Caco-2 cells were determined. Metabolite extractions of the cell cultures were conducted and samples were analyzed by HPLC MS/MS to investigate the metabolic difference of the host cells with or without addition of phenolic compounds.