Browsing Lee, Richard by Title
Now showing items 17-32 of 32
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Ice nucleating active bacteria reduce the cold-hardiness of the freeze-intolerant Colorado potato beetle (Coleoptera: Chrysomelidae).
In laboratory experiments, a freeze-dried concentrated form of the icenucleating active bacteria, Pseudomonas syringae, was used to decrease the supercooling capacity of field-collected diapausing Colorado potato beetles, ... -
Ice nuclei in soil compromise cold hardiness of hatchling painted turtles, Chrysemys picta.
Hatchling painted turtles (Chrysemys picta) commonly overwinter within their natal nests and survive exposure to temperatures as low as -12 degrees C by supercooling. We report that the supercooling capacity of hatchling ... -
Ice-nucleating active bacteria decrease the cold-hardiness of stored grain insects.
This report provides further evidence that a freeze-dried, concentrated form of Pseudomonas syringae, an ice-nucleating active bacteria, reduces the cold tolerance of stored grain insect pests. Application of ice-nucleating ... -
Identification and expression of a putative facilitative urea transporter in three species of true frogs (Ranidae): implications for terrestrial adaptation.
Urea transporters (UTs) help mediate the transmembrane movement of urea and therefore are likely important in amphibian osmoregulation. Although UTs contribute to urea reabsorption in anuran excretory organs, little is ... -
Identification of ice nucleating active Pseudomonas fluorescens strains for biological control of overwintering Colorado potato beetles (Coleoptera: Chrysomelidae).
Laboratory studies were conducted to identify ice-nucleating active bacterial strains able to elevate the supercooling point, the temperature at which freezing is initiated in body fluids, of Colorado potato beetles, ... -
Inoculative freezing and the problem of winter survival for freshwater macroinvertebrates
Due to the thermal buffering of their environment, aquatic invertebrates are less likely than their terrestrial counterparts to face temperatures substantially below 0°C. Aquatic invertebrates may not be able to avoid ... -
Low temperature acclimation in the desert spider, Agelenopsis aperta.
Agelenopsis aperta (Gertsch) inhabits desert grasslands and lava beds in the southwestern U.S.A. The capacity of this species to cold-harden was assessed by exposing second generation laboratory- reared specimens to an ... -
Physiological Ecology of Overwintering in the Hatchling Painted Turtle: Multiple-Scale Variation in response to Environmental Stress
We integrates field and laboratory studies in an investigation of water balance, energy use, and mechanisms of cold-hardiness in hatchling painted turtles (Chrysemys picta) indigenous to west-central Nebraska (Chrysemys ... -
Postfreeze reduction of locomotor endurance in the freeze-tolerant wood frog, Rana sylvatica.
Considerable study has focused on the physiological adaptations for freeze tolerance in the wood frog, Rana sylvatica, a northern species that overwinters within the frost zone, but little attention has been paid to the ... -
Seasonal variation in the hepatoproteome of the dehydration- and freeze-tolerant wood frog, Rana sylvatica
Winter’s advent invokes physiological adjustments that permit temperate ectotherms to cope with stresses such as food shortage, water deprivation, hypoxia, and hypothermia. We used liquid chromatography (LC) in combination ... -
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica
We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive ... -
Survival and physiological responses of hatchling Blanding's turtles (Emydoidea blandingii) to submergence in normoxic and hypoxic water under simulated winter conditions.
Abstract Overwintering habits of hatchling Blanding’s turtles (Emydoidea blandingii) are unknown. To determine whether these turtles are able to survive winter in aquatic habitats, we submerged hatchlings in normoxic (155 ... -
Surviving the big chill: overwintering strategies of aquatic and terrestrial insects.
The purpose of this paper is to describe the cold-hardiness of aquatic insects and to use the literature to compare physiological and behavioral strategies that aquatic and terrestrial insects use to cope with minimum ... -
Topical application of ice-nucleating-active bacteria decreases insect cold tolerance
The majority of overwintering insects avoid lethal freezing by lowering the temperature at which ice spontaneously nucleates within their body fluids. We examined the effect of ice-nucleating-active bacteria on the ... -
Using microrespirometers to measure oxygen consumption by insects and small invertebrates.
A variety of physiological studies require the measurement of oxygen consumption. Unfortunately, the techniques for measuring respiration rate often require expensive equipment or difficult procedures that are not available ... -
Winter biology & freeze tolerance in the goldenrod gall fly
Birds migrate. Bears hibernate. Turtles and frogs retreat to the bottom of lakes. Most animals must avoid harsh winter conditions; few can survive freezing. Larvae of the goldenrod gall fly (Eurosta solidaginis), can ...